Wang I-Fan, Reddy Narsa M, Shen C-K James
Institute of Molecular Biology, Academia Sinica, National Taiwan University, Taipei, Taiwan 115, Republic of China.
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13583-8. doi: 10.1073/pnas.212483099. Epub 2002 Oct 2.
The nuclei of eukaryotic cells consist of discrete substructures. These substructures include the nuclear bodies, which have been implicated in a number of biological processes such as transcription and splicing. However, for most nuclear bodies, the details of involvement in these processes in relation to their three-dimensional distributions in the nucleus are still unclear. Through the analysis of TDP, a protein functional in both transcriptional repression and alternative splicing, we have identified a new category of nuclear bodies within which the TDP molecules reside. Remarkably, the TDP bodies (TBs) colocalize or overlap with several different types of nuclear bodies previously suggested to function in transcription or splicing. Of these nuclear bodies, the Gemini of coiled bodies (GEM) seems to associate with TB through the interaction between survival motor neuron (SMN) protein and TDP. Furthermore, TB sometimes appears to be the bridge of two or more of these other nuclear bodies. Our data suggest the existence of a hierarchy and possibly functional arrangement of the nuclear bodies within the eukaryotic nuclei.
真核细胞的细胞核由离散的亚结构组成。这些亚结构包括核体，它们与许多生物学过程有关，如转录和剪接。然而，对于大多数核体而言，它们在细胞核中的三维分布与这些过程的具体关联细节仍不清楚。通过对在转录抑制和可变剪接中均起作用的蛋白质TDP进行分析，我们鉴定出了一类新的核体，TDP分子存在于其中。值得注意的是，TDP体（TBs）与先前认为在转录或剪接中起作用的几种不同类型的核体共定位或重叠。在这些核体中，卷曲体的双子体（GEM）似乎通过生存运动神经元（SMN）蛋白与TDP之间的相互作用与TB相关联。此外，TB有时似乎是两个或更多其他这些核体之间的桥梁。我们的数据表明真核细胞核内存在核体的层次结构以及可能的功能排列。
