Cosgrove Andrew J, Nieduszynski Conrad A, Donaldson Anne D
Cancer Research UK Chromosome Replication Research Group, Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland.
Genes Dev. 2002 Oct 1;16(19):2485-90. doi: 10.1101/gad.231602.
We have investigated whether the Ku complex is involved in regulating DNA replication in the yeast Saccharomyces cerevisiae. We find that Ku proteins control the replication time of telomeric regions; replication origins located close to telomeres or within subtelomeric repeat sequences normally initiate late, but are activated much earlier in mutants lacking Ku function. In contrast, origins distant from telomeres initiate replication at the normal time. Ku is one of the first components identified as important for replication timing, and specification of the replication time of chromosome ends by Ku is consistent with its role in maintaining telomere localization.
我们研究了Ku复合物是否参与调控酿酒酵母中的DNA复制。我们发现Ku蛋白控制端粒区域的复制时间；位于靠近端粒或亚端粒重复序列内的复制起点通常启动较晚，但在缺乏Ku功能的突变体中会更早被激活。相比之下，远离端粒的起点在正常时间启动复制。Ku是最早被确定对复制时间重要的成分之一，并且Ku对染色体末端复制时间的特异性与其在维持端粒定位中的作用一致。
