Ramirez R I, Tiku M L, Ogra P L
J Clin Microbiol. 1975 Feb;1(2):185-7. doi: 10.1128/jcm.1.2.185-187.1975.
A recently described hemagglutination test for hepatitis B antigen (HBsAg) using turkey erythrocytes coated with horse antibody to HBsAg purified by affinity column chromatography was evaluated on a comparative basis with 100 HBsAg-positive and -negative serum samples. The turkey erythrocyte hemagglutination test (TEHA) was found to be less sensitive than radioimmunoassay (RIA) but gave far better results than counterimmunoelectrophoresis. Quantitative titration of HBsAg in serial dilutions of the samples appeared to be more reliably performed by TEHA than by RIA. TEHA is a simple and sensitive technique for the detection of HBsAg and may offer several practical advantages over RIA.
采用经亲和柱层析纯化的抗乙型肝炎表面抗原(HBsAg)马抗体包被的火鸡红细胞,对最近描述的一种检测HBsAg的血凝试验进行了评估,并与100份HBsAg阳性和阴性血清样本进行了比较。结果发现,火鸡红细胞血凝试验(TEHA)不如放射免疫测定法(RIA)敏感,但比对流免疫电泳效果好得多。与RIA相比,TEHA对样本系列稀释液中的HBsAg进行定量滴定似乎更可靠。TEHA是一种检测HBsAg的简单且灵敏的技术,与RIA相比可能具有若干实际优势。