Reyna-Neyra Andrea, Camacho-Arroyo Ignacio, Ferrera Patricia, Arias Clorinda
Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF, México, México.
Brain Res Bull. 2002 Sep 30;58(6):607-12. doi: 10.1016/s0361-9230(02)00829-8.
The molecular mechanisms involved in the regulation of synaptic plasticity and neuroprotection by estradiol (E(2)) and progesterone (P(4)) are unknown. Because these processes involve changes in cytoskeleton organization, we studied the effects of E(2) and P(4) in the expression of two cytoskeletal proteins: microtubule associated protein 2 (MAP2) and tau in the hippocampus and the frontal cortex of ovariectomized adult rats. While tau expression was unaffected by E(2) and P(4), an increase in MAP2 protein content in the hippocampus but not in the cortex was observed after E(2) and P(4) treatments. Interestingly, these steroids did not modify MAP2 mRNA content in the hippocampus. These data suggest that MAP2 is involved in the structural changes induced by E(2) and P(4) in the rat hippocampus, and that MAP2 expression is regulated by these steroid hormones at a postranscriptional level.
雌二醇（E₂）和孕酮（P₄）调节突触可塑性和神经保护作用的分子机制尚不清楚。由于这些过程涉及细胞骨架组织的变化，我们研究了E₂和P₄对成年去卵巢大鼠海马体和额叶皮质中两种细胞骨架蛋白表达的影响：微管相关蛋白2（MAP2）和tau蛋白。虽然tau蛋白的表达不受E₂和P₄的影响，但在E₂和P₄处理后，海马体中MAP2蛋白含量增加，而皮质中未观察到这种增加。有趣的是，这些类固醇并未改变海马体中MAP2 mRNA的含量。这些数据表明，MAP2参与了E₂和P₄在大鼠海马体中诱导的结构变化，并且MAP2的表达在转录后水平受到这些类固醇激素的调节。
