School of Medicine, University of St. Andrews, North Haugh, St. Andrews KY16 9TF, UK.
Nat Commun. 2012;3:1027. doi: 10.1038/ncomms2024.
There has been an immense drive in modern microscopy towards miniaturization and fibre-based technology. This has been necessitated by the need to access hostile or difficult environments in situ and in vivo. Strategies to date have included the use of specialist fibres and miniaturized scanning systems accompanied by ingenious microfabricated lenses. Here we present a novel approach for this field by utilizing disordered light within a standard multimode optical fibre for lensless microscopy and optical mode conversion. We demonstrate the modalities of bright- and dark-field imaging and scanning fluorescence microscopy at acquisition rates that allow observation of dynamic processes such as Brownian motion of mesoscopic particles. Furthermore, we show how such control can realize a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre-based implementations of super-resolution or light-sheet microscopy.
现代显微镜技术朝着微型化和光纤技术方向发展，这是因为需要在原位和体内进入恶劣或困难的环境。迄今为止，已有多种策略，包括使用特殊光纤和微型扫描系统，以及巧妙的微加工透镜。在这里，我们通过在标准多模光纤中利用无序光来实现无透镜显微镜和光学模式转换，为该领域提供了一种新的方法。我们演示了明场和暗场成像以及扫描荧光显微镜的模式，其采集速度允许观察诸如介观粒子的布朗运动等动态过程。此外，我们展示了如何通过这种控制来实现新型模式转换器，并生成各种类型的先进光场，如无传播光束和光涡旋。这些可能对未来基于光纤的超分辨率或光片显微镜的实现有用。
