伯氏疏螺旋体gyrB突变体中的groEL表达。
groEL expression in gyrB mutants of Borrelia burgdorferi.
作者信息
Alverson Janet, Samuels D Scott
机构信息
Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA.
出版信息
J Bacteriol. 2002 Nov;184(21):6069-72. doi: 10.1128/JB.183.21.6069-6072.2002.
Abstract
GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.
摘要
GroEL蛋白和groEL mRNA转录本在莱姆病病原体伯氏疏螺旋体的gyrB突变体中上调。此外，gyrB突变体中的蛋白质和转录本水平高于野生型伯氏疏螺旋体实验性热休克培养物中的水平。尽管groEL位于伯氏疏螺旋体的线性染色体上，但gyrB突变体中的环状DNA比野生型细胞中的更松弛。据我们所知，这是关于细菌中DNA拓扑结构对线性DNA分子基因表达影响的首个证据，尽管是间接证据。
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