Lu Xiaoquan, Zhu Kaimei, Zhang Min, Liu Hongde, Kang Jingwan
Department of Chemistry, Northwest Normal University, Lanzhou 730070, Gansu, China.
J Biochem Biophys Methods. 2002 Aug-Sep;52(3):189-200. doi: 10.1016/s0165-022x(02)00074-x.
The interaction of the two new synthesized transition-metal complexes, ML(2) (M=Co, Cu, L=1,8-dihydroxyethyl-1, 3,8,10,13-hexa-azacyclotetradecane) with calf thymus DNA was probed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Adding deoxyribonucleic acid (DNA) into CoL and CuL solution, the i(p) value of all the peaks of CoL and CuL significantly decreased in proportion to concentration of DNA. Glassy carbon electrodes (GCEs) were modified with DNA by adsorption, and it was electrochemically characterized with transition-metal complexes, ML. The DNA modification layer on the GCE is unstable to alkali and to heat, but stable to acid solutions and very stable in long stock in a dry state. It could be seen that peak potential shifted positively and the peak current increased significantly. The electrochemical parameters, binding constant (k(n+)) and binding sites(s) were calculated by a nonlinear regression method.
通过循环伏安法（CV）和差分脉冲伏安法（DPV）探究了两种新合成的过渡金属配合物ML₂（M = Co、Cu，L = 1,8 - 二羟乙基 - 1,3,8,10,13 - 六氮杂环十四烷）与小牛胸腺DNA的相互作用。向[CoL]²⁺和[CuL]²⁺溶液中加入脱氧核糖核酸（DNA），[CoL]²⁺和[CuL]²⁺所有峰的i(p)值均随DNA浓度的增加而显著降低。通过吸附法用DNA修饰玻碳电极（GCE），并用过渡金属配合物[ML]²⁺对其进行电化学表征。GCE上的DNA修饰层对碱和热不稳定，但对酸性溶液稳定，在干燥状态下长期保存非常稳定。可以看出，峰电位正向移动且峰电流显著增加。通过非线性回归方法计算了电化学参数、结合常数（k(n+)）和结合位点（s）。
