Budowle Bruce, Garofano Paolo, Hellman Andreas, Ketchum Melba, Kanthaswamy Sree, Parson Walther, van Haeringen Wim, Fain Steve, Broad Tom
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA.
Int J Legal Med. 2005 Sep;119(5):295-302. doi: 10.1007/s00414-005-0545-9. Epub 2005 Apr 16.
Genetic analysis in animals has been used for many applications, such as kinship analysis, for determining the sire of an offspring when a female has been exposed to multiple males, determining parentage when an animal switches offspring with another dam, extended lineage reconstruction, estimating inbreeding, identification in breed registries, and speciation. It now also is being used increasingly to characterize animal materials in forensic cases. As such, it is important to operate under a set of minimum guidelines that assures that all service providers have a template to follow for quality practices. None have been delineated for animal genetic identity testing. Based on the model for human DNA forensic analyses, a basic discussion of the issues and guidelines is provided for animal testing to include analytical practices, data evaluation, nomenclature, allele designation, statistics, validation, proficiency testing, lineage markers, casework files, and reporting. These should provide a basis for professional societies and/or working groups to establish more formalized recommendations.
动物遗传分析已被用于许多应用,如亲缘关系分析,用于在雌性与多个雄性交配时确定后代的父系,在动物与另一只母畜交换后代时确定亲子关系,扩展谱系重建,估计近亲繁殖,品种登记中的识别,以及物种形成。现在它也越来越多地用于法医案件中动物材料的特征鉴定。因此,按照一套最低指导方针进行操作很重要,以确保所有服务提供商都有一个质量规范的模板可遵循。目前还没有针对动物遗传身份检测制定相关指导方针。基于人类DNA法医分析模型,本文对动物检测的问题和指导方针进行了基本讨论,包括分析方法、数据评估、命名法、等位基因指定、统计学、验证、能力验证、谱系标记、案例文件和报告。这些应能为专业协会和/或工作组制定更正式的建议提供基础。