Kanthaswamy Sree, Tom Bradley K, Mattila Anna-Maria, Johnston Eric, Dayton Melody, Kinaga Jennifer, Erickson Bethany Joy-Alise, Halverson Joy, Fantin Dennis, DeNise Sue, Kou Alexander, Malladi Venkat, Satkoski Jessica, Budowle Bruce, Smith David Glenn, Koskinen Mikko T
California National Primate Research Center, University of California, Davis, CA 95616, USA.
J Forensic Sci. 2009 Jul;54(4):829-40. doi: 10.1111/j.1556-4029.2009.01080.x. Epub 2009 May 26.
Canine biological specimens are often part of the physical evidence from crime scenes. Until now, there have been no validated canine-specific forensic reagent kits available. A multiplex genotyping system, comprising 18 short tandem repeats (STRs) and a sex-linked zinc finger locus for gender determination, was developed for generating population genetic data assessing the weight of canine forensic DNA profiles. Allele frequencies were estimated for 236 pedigreed and 431 mixed breed dogs residing in the U.S. Average random match probability is 1 in 2 x 10(33) using the regional database and 1 in 4 x 10(39) using the breed dataset. Each pedigreed population was genetically distinct and could be differentiated from the mixed breed dog population but genetic variation was not significantly correlated with geographic transition. Results herein support the use of the allele frequency data with the canine STR multiplex for conveying the significance of identity testing for forensic casework, parentage testing, and breed assignments.
犬类生物样本常常是犯罪现场物证的一部分。到目前为止，还没有经过验证的针对犬类的法医试剂试剂盒。开发了一种多重基因分型系统，该系统包含18个短串联重复序列（STR）和一个用于性别鉴定的性连锁锌指基因座，用于生成群体遗传数据，以评估犬类法医DNA图谱的权重。估计了居住在美国的236只纯种犬和431只混种犬的等位基因频率。使用区域数据库时，平均随机匹配概率为1/2×10³³，使用品种数据集时为1/4×10³⁹。每个纯种群体在基因上都是独特的，并且可以与混种犬群体区分开来，但遗传变异与地理过渡没有显著相关性。本文的结果支持使用等位基因频率数据与犬类STR多重检测，以传达法医案件工作、亲子鉴定和品种鉴定中身份测试的重要性。
