咖啡因可诱导人神经母细胞瘤细胞系SK-N-MC发生凋亡。
Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC.
作者信息
Jang Mi-Hyeon, Shin Min-Chul, Kang In-Sug, Baik Hyung-Hwan, Cho Yong-Ho, Chu Jong-Phill, Kim Ee-Hwa, Kim Chang-Ju
机构信息
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
出版信息
J Korean Med Sci. 2002 Oct;17(5):674-8. doi: 10.3346/jkms.2002.17.5.674.
Caffeine is one of the most widely consumed neuroactive drugs, coming mostly from everyday beverages such as coffee and tea. To investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, DNA fragmentation assay, and caspase-3 enzyme assay were performed on SK-N-MC human neuroblastoma cells. Cells treated with caffeine at concentrations as high as 10 mM exhibited several characteristics of apoptosis. In addition, caffeine was shown to increase the caspase-3 activity. These results suggest that high-dose of caffeine induces apoptosis in human neuroblastoma cells, probably by increasing the caspase-3 enzyme activity.
咖啡因是消费最为广泛的神经活性药物之一，主要来源于咖啡和茶等日常饮品。为研究咖啡因是否会在中枢神经系统中诱导细胞凋亡，对SK-N-MC人神经母细胞瘤细胞进行了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测、4,6-二脒基-2-苯基吲哚（DAPI）染色、末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）检测、流式细胞术分析、DNA片段化检测以及半胱天冬酶-3酶活性检测。用高达10 mM浓度的咖啡因处理的细胞表现出了细胞凋亡的若干特征。此外，咖啡因还被证明会增加半胱天冬酶-3的活性。这些结果表明，高剂量咖啡因可能通过增加半胱天冬酶-3酶的活性，诱导人神经母细胞瘤细胞凋亡。
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