Perumal Angalammal, AlSalhi Mohamad S, Kanakarajan Sivakumari, Devanesan Sandhanasamy, Selvaraj Rajesh, Tamizhazhagan Vairakannu
Department of Zoology, Presidency College, Chennai 600005, India.
Department of Physics and Astronomy, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Mar;28(3):1816-1825. doi: 10.1016/j.sjbs.2020.12.027. Epub 2020 Dec 21.
The current investigation was taken to screen the phytoconstituents present in fruit endocarp various extracts of commonly called as Rambutan fruit and its anticancer property against human hepatocellular carcinoma (HepG-2) cells. Different analytical techniques including qualitative phytochemical analysis, cell viability assay (MTT), apoptotic nuclear staining (DAPI), DNA fragmentation assay, Attenuated total reflection (ATR) and Gas chromatography-mass spectrometry (GC-MS) spectral analysis were carried out. ATR and GC-MS study revealed the presence of functional groups and 9 compounds, respectively in methanol endocarp extract. The results obtained depicts that methanol endocarp extract profoundly controlled cell proliferation and caused shrinkage of HepG-2 cells from polygonal to spherical shape. DAPI staining revealed that methanol endocarp extract caused increased fragmentation of nucleus and DNA fragmentation, which can be taken as a sign of apoptosis. The anticancer potential of methanol fruit endocarp extract of than other extracts and could be used successfully in future drug delivery systems and other biomedical concerns.
目前的研究旨在筛选俗称红毛丹果实的果内果皮各种提取物中存在的植物成分及其对人肝癌（HepG-2）细胞的抗癌特性。采用了不同的分析技术，包括定性植物化学分析、细胞活力测定（MTT）、凋亡核染色（DAPI）、DNA片段化测定、衰减全反射（ATR）和气相色谱-质谱（GC-MS）光谱分析。ATR和GC-MS研究分别揭示了甲醇果内果皮提取物中官能团和9种化合物的存在。所得结果表明，甲醇果内果皮提取物能显著控制细胞增殖，并使HepG-2细胞从多边形收缩为球形。DAPI染色显示，甲醇果内果皮提取物导致细胞核碎片化增加和DNA片段化，这可被视为细胞凋亡的迹象。甲醇果内果皮提取物的抗癌潜力优于其他提取物，未来可成功用于药物递送系统和其他生物医学领域。
