Bednarek Piotr T, Lewandowska Renata, Kubicka Helena, Masojć Piotr
The Botanical Garden - the Centre for the Conservation of Biological Diversity of the Polish Academy of Sciences, 02-973 Warsaw, ul. Prawdziwka 2, Poland.
Cell Mol Biol Lett. 2002;7(2B):721-36.
Twenty-five AFLPs, previously linked to fertility restoration genes for the male-sterilizing PAMPA cytoplasm (cms-P) in a restricted rye population, were studied in an enlarged population of 120 plants. A strong association with the trait was verified for 19 of the markers. The recombination of these markers was tested and two linkage groups were identified: one consisting of six and the other of eight AFLPs. The remaining markers were segregated as independent loci. Using wheat-rye addition and substitution lines, the AFLPs were assigned to individual rye chromosomes. AFLP profiles of such lines were generated to identify the DNA fragments co-migrating with individual markers. This identified 1R and 3R as the two chromosomes corresponding to the linkage groups of eight and six markers, respectively. Mapping in a DS2 x RXL10 population linked four additional AFLPs to chromosome arms 1RS, 3RL, 4RL and 6RL. RAPD and SSR markers mapped in various populations and known to be located on the appropriate chromosomes did not disrupt the C394-F2 population into sterile and fertile phenotypes. It was concluded that the identified markers would reduce by one half the number of primer pair combinations needed for molecular breeding programs or for the selection of parental forms for rye hybrid crosses.
在一个由120株植物组成的扩大群体中，研究了先前在一个有限的黑麦群体中与雄性不育的PAMPA细胞质（cms - P）育性恢复基因相关联的25个扩增片段长度多态性（AFLP）。对其中19个标记与该性状的强关联进行了验证。对这些标记的重组进行了测试，鉴定出两个连锁群：一个由6个AFLP组成，另一个由8个AFLP组成。其余标记作为独立位点分离。利用小麦 - 黑麦附加系和代换系，将AFLP定位到各个黑麦染色体上。生成这些系的AFLP图谱以鉴定与各个标记共迁移的DNA片段。这确定了1R和3R分别是对应于由8个和6个标记组成的连锁群的两条染色体。在DS2×RXL10群体中的定位将另外4个AFLP连接到染色体臂1RS、3RL、4RL和6RL上。在不同群体中定位且已知位于适当染色体上的随机扩增多态性DNA（RAPD）和简单序列重复（SSR）标记并未将C394 - F2群体分离为不育和可育表型。得出的结论是，所鉴定的标记将使分子育种计划或黑麦杂交亲本形式选择所需的引物对组合数量减少一半。
