Stojałowski Stefan, Jaciubek Milosława, Masojć Piotr
Department of Genetics and Plant Breeding, Agricultural University, Slowackiego 17, 71-437 Szczecin, Poland.
J Appl Genet. 2005;46(4):371-3.
The study aimed at testing the usefulness of recently developed SCAR markers on rye (Secale cereale L.) chromosome 4R in hybrid breeding based on the C source of male sterility-inducing cytoplasm. Of 10 markers studied, 4 revealed polymorphisms between 2 inbred lines (544cms-C and Ot0-20) crossed to develop F2 and BC1 mapping populations. Analyses performed on 94 F2 and 93 BC1 plants allowed to extend a formerly constructed genetic map of chromosome arm 4RL. Three SCAR markers (SCP14M55, SCP15M55 and SCP16M58) were mapped in the vicinity of gene Rfc1, which restores male fertility in the C cytoplasm. The 3 tested SCAR markers proved to be effective in marker-assisted selection (MAS) for male fertility/sterility.
该研究旨在基于雄性不育诱导细胞质的C源，测试最近开发的黑麦（Secale cereale L.）4R染色体上的SCAR标记在杂交育种中的实用性。在研究的10个标记中，4个在用于构建F2和BC1作图群体的2个自交系（544cms-C和Ot0-20）之间显示出多态性。对94株F2和93株BC1植株进行的分析扩展了先前构建的4RL染色体臂遗传图谱。三个SCAR标记（SCP14M55、SCP15M55和SCP16M58）被定位在恢复C细胞质中雄性育性的Rfc1基因附近。经测试，这3个SCAR标记在雄性育性/不育性的标记辅助选择（MAS）中被证明是有效的。
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