Page G I, Davies S J
Fish Nutrition Unit, Department of Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Comp Biochem Physiol C Toxicol Pharmacol. 2002 Nov;133(3):443-51. doi: 10.1016/s1532-0456(02)00168-0.
This study was designed to assess the effects of dietary carotenoid supplementation on liver and kidney xenobiotic-metabolizing enzymes in the rainbow trout. Twelve rainbow trout (mean weight 266+/-10 g) were assigned to each of three replicate tanks for each of four dietary treatments; astaxanthin, canthaxanthin, negative control and positive control using beta-naphthoflavone, at a target dietary inclusion of 100 mg kg(-1) for each additive. Fish were fed for 3 weeks at a level of 1.2% body wt. day(-1). Serum carotenoid levels were used as indicators of exposure and were not significantly different (P>0.05) between carotenoid-fed trout. Livers and kidney were frozen separately in liquid N(2) by immersion and microsomal fractions from pooled samples (n=3) assayed for xenobiotic-metabolizing enzyme (cytochrome P450 monoxygenase) activities including ethoxyresorufin O-deethylase; methoxyresorufin O-demethylase; pentoxyresorufin O-dealkylase; benzoxyresorufin O-dearylase; and the conjugating enzymes glucuronosyl transferase; and glutathione-s-transferase. Results revealed that carotenoid treatment did not significantly (P>0.05) induce any enzyme system examined. Results are discussed in the context of metabolism of absorbed carotenoids.
本研究旨在评估日粮中添加类胡萝卜素对虹鳟鱼肝和肾中异生素代谢酶的影响。将12条虹鳟（平均体重266±10克）分配到三个重复水箱中，每个水箱对应四种日粮处理中的一种；虾青素、角黄素、阴性对照和使用β-萘黄酮的阳性对照，每种添加剂的目标日粮添加量为100毫克/千克。以鱼体重1.2%的水平投喂3周。血清类胡萝卜素水平用作暴露指标，类胡萝卜素喂养的虹鳟之间无显著差异（P>0.05）。肝脏和肾脏通过浸没分别在液氮中冷冻，合并样本（n=3）的微粒体部分用于检测异生素代谢酶（细胞色素P450单加氧酶）活性，包括乙氧异吩唑酮O-脱乙基酶、甲氧基异吩唑酮O-脱甲基酶、戊氧基异吩唑酮O-脱烷基酶、苯氧基异吩唑酮O-脱芳基酶，以及结合酶葡萄糖醛酸转移酶和谷胱甘肽-S-转移酶。结果显示，类胡萝卜素处理未显著（P>0.05）诱导所检测的任何酶系统。在吸收的类胡萝卜素代谢背景下对结果进行了讨论。
