Chew B P, Wong M W, Park J S, Wong T S
Department of Animal Sciences, Washington State University, Pullman 99164, USA.
Anticancer Res. 1999 Nov-Dec;19(6B):5223-7.
The in vivo modulatory effect of beta-carotene, astaxanthin and canthaxanthin on lymphocyte function was investigated. Female BALB/c mice (8 wk old) were fed a basal diet containing 0, 0.1% or 0.4% beta-carotene, astaxanthin or canthaxanthin for 0, 2 or 4 wk (n = 8/diet/period). Splenic lymphocytes were isolated and mitogen-stimulated proliferation, IL-2 production and lymphocyte cytotoxicity were assessed. Body weight and feed intake were not different among dietary treatments. Plasma carotenoids were undetectable in unsupplemented mice but concentrations of the respective carotenoids were elevated in mice fed 0.1 or 0.4% beta-carotene (0.22 and 0.39 mumol/L), astaxanthin (16.4 and 50.2 mumol/L) and canthaxanthin (5.00 and 7.02 mumol/L) respectively. Mice fed both dietary levels of beta-carotene and astaxanthin had enhanced phytohemagglutinin-induced lymphoblastogenesis compared to unsupplemented mice (P < 0.03). No treatment difference was detected with concanavalin A- or lipopolysaccharide-induced lympho-proliferation nor with IL-2 production (P < 0.05). Astaxanthin (0.1%) also enhanced lymphocyte cytotoxic activity (P < 0.08). In contrast, canthaxanthin did not significantly influence any of the lymphocyte functions measured. Results indicate that beta-carotene and astaxanthin but not canthaxanthin exert enhanced splenic lymphocyte function in mice.
研究了β-胡萝卜素、虾青素和角黄素对淋巴细胞功能的体内调节作用。选用8周龄雌性BALB/c小鼠，分别饲喂含0、0.1%或0.4%β-胡萝卜素、虾青素或角黄素的基础日粮0、2或4周（每组日粮每个时间段n = 8）。分离脾淋巴细胞，评估丝裂原刺激的增殖、IL-2产生和淋巴细胞细胞毒性。不同日粮处理组之间的体重和采食量没有差异。未补充的小鼠血浆中未检测到类胡萝卜素，但饲喂0.1%或0.4%β-胡萝卜素（0.22和0.39μmol/L）、虾青素（16.4和50.2μmol/L）和角黄素（5.00和7.02μmol/L）的小鼠中，各自类胡萝卜素的浓度升高。与未补充的小鼠相比，饲喂两种日粮水平β-胡萝卜素和虾青素的小鼠，其植物血凝素诱导的淋巴细胞生成增强（P < 0.03）。伴刀豆球蛋白A或脂多糖诱导的淋巴细胞增殖以及IL-2产生方面未检测到处理差异（P < 0.05）。虾青素（0.1%）也增强了淋巴细胞细胞毒性活性（P < 0.08）。相比之下，角黄素对所测的任何淋巴细胞功能均无显著影响。结果表明，β-胡萝卜素和虾青素而非角黄素可增强小鼠脾淋巴细胞功能。
