Bergamini A, Cepparulo M, Bolacchi F, Araco A, Tisone G, Ombres D, Rocchi G, Angelico M
Department of Public Health and Cellular Biology, Chair of Infectious Diseases, University of Rome Tor Vergata, Rome, Italy.
Clin Exp Immunol. 2002 Nov;130(2):293-9. doi: 10.1046/j.1365-2249.2002.01970.x.
Here, CD40L expression and cytokine production have been analysed in peripheral blood cells from orthotopic liver transplantation (OLT) recipients treated with ribavirin for recurrent chronic hepatitis C. The study included 18 OLT recipients treated with ribavirin, eight control OLT recipients and 10 healthy controls. FACS analysis showed that baseline expression of CD40L was not different between ribavirin-treated patients and controls. In contrast, after stimulation with both HCV core antigen and phorbol myristate acetate (PMA) plus ionomycin (IO), the expression of CD40L on CD4 lymphocytes was significantly higher in the ribavirin group compared with controls. In the ribavirin group, the increased expression of CD40L significantly correlated with reduction of HCV RNA levels with respect to pretreatment values. Finally, ribavirin treatment was not associated with modification of PMA-IO-induced cytokine production by T lymphocytes and interleukin (IL)-1beta and tumour necrosis-alpha (TNF)-alpha production by CD40L-stimulated monocytes. In conclusion, these data indicate that ribavirin -upmodulates CD40L expression on CD4 T cells, a property which may account in part for its ability to enhance the antiviral activity of interferon-alpha in the treatment of chronic HCV infection.
在此，我们分析了接受利巴韦林治疗复发性慢性丙型肝炎的原位肝移植（OLT）受者外周血细胞中的CD40L表达和细胞因子产生情况。该研究纳入了18例接受利巴韦林治疗的OLT受者、8例对照OLT受者和10例健康对照。流式细胞术分析显示，利巴韦林治疗组患者和对照组之间CD40L的基线表达无差异。相比之下，在用丙型肝炎病毒核心抗原以及佛波酯肉豆蔻酸酯乙酸盐（PMA）加离子霉素（IO）刺激后，利巴韦林组CD4淋巴细胞上CD40L的表达显著高于对照组。在利巴韦林组中，CD40L表达的增加与相对于治疗前值的丙型肝炎病毒RNA水平降低显著相关。最后，利巴韦林治疗与T淋巴细胞经PMA-IO诱导的细胞因子产生以及CD40L刺激的单核细胞产生白细胞介素（IL）-1β和肿瘤坏死因子-α（TNF-α）的改变无关。总之，这些数据表明利巴韦林上调CD4 T细胞上的CD40L表达，这一特性可能部分解释了其在慢性丙型肝炎感染治疗中增强α干扰素抗病毒活性的能力。
