Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
College of Natural, Applied and Health Sciences, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, Zhejiang Province, China.
Biomolecules. 2018 Mar 23;8(2):15. doi: 10.3390/biom8020015.
Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant A in , and a recombinant AtClAP fragment (AtClAP) can produce cyclic adenosine 3',5' monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.
腺苷酸环化酶（ACs），与鸟苷酸环化酶（GCs）类似，越来越被认为是许多植物过程的重要组成部分，包括生物和非生物胁迫反应。为了鉴定新的 ACs，我们应用了一个源自经过实验测试的 GCs 的搜索基序，并鉴定了许多候选者，包括网格蛋白组装蛋白（AT1G68110；AtClAP）。AtClAP 包含一个催化中心，可以补充 AC 缺陷突变体 A 在中的功能，并且重组 AtClAP 片段（AtClAP）可以在体外从三磷酸腺苷（ATP）产生环腺苷酸 3'，5' 一磷酸（cAMP）。此外，基因表达和表达相关性的综合分析表明 cAMP 参与了病原体防御以及内吞作用过程中肌动蛋白细胞骨架的重塑。
