Lombaert G A, Pellaers P, Chettiar M, Lavalee D, Scott P M, Lau B P-Y
Health Canada, Health Products and Food Branch, 510 Lagimodière Blvd, Winnipeg, MB, R2J 3Y1, Canada.
Food Addit Contam. 2002 Sep;19(9):869-77. doi: 10.1080/02652030210145027.
One-hundred and one specimens of coffee were gathered from retail outlets across Canada and analysed for ochratoxin A. Seventy-one specimens were roasted beans or roasted ground coffee, and 30 were instant (or 'soluble') coffees. All samples were extracted with methanol-sodium bicarbonate. The extracts were cleaned up either by immunoaffinity column chromatography or by a combination of solid-phase extraction and immunoaffinity column chromatography. Ochratoxin A was quantified by liquid chromatography (LC) with fluorescence detection. The minimum quantifiable level was 0.1 ng g(-1). Ochratoxin A was present, above the minimum quantifiable level, in 42 (59%) of 71 beans and ground coffee and in 20 (67%) of 30 instant coffees. The mean ochratoxin A level in the positive samples of beans and ground coffee was 0.6 ng g(-1), and the mean level in the positive samples of instant coffee was 1.1 ng g(-1).
从加拿大各地的零售商店收集了101份咖啡样本，并对其进行赭曲霉毒素A分析。71份样本为烘焙咖啡豆或研磨咖啡，30份为速溶（或“可溶”）咖啡。所有样品均用甲醇 - 碳酸氢钠进行提取。提取物通过免疫亲和柱色谱法或固相萃取与免疫亲和柱色谱法相结合的方法进行净化。赭曲霉毒素A通过带荧光检测的液相色谱法（LC）进行定量。最低可定量水平为0.1 ng g(-1)。在71份咖啡豆和研磨咖啡中，有42份（59%）赭曲霉毒素A含量高于最低可定量水平；在30份速溶咖啡中，有20份（67%）高于该水平。咖啡豆和研磨咖啡阳性样本中赭曲霉毒素A的平均含量为0.6 ng g(-1)，速溶咖啡阳性样本中的平均含量为1.1 ng g(-1)。
