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生咖啡豆、烘焙咖啡豆及其相应副产物中赭曲霉毒素A的天然存在情况与抗氧化活性。

Natural occurrence of ochratoxin A and antioxidant activities of green and roasted coffees and corresponding byproducts.

作者信息

Napolitano Aurora, Fogliano Vincenzo, Tafuri Alessio, Ritieni Alberto

机构信息

Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Via Università 100, Parco Gussone, 80055 Portici, Napoli, Italy.

出版信息

J Agric Food Chem. 2007 Dec 12;55(25):10499-504. doi: 10.1021/jf071959+. Epub 2007 Nov 20.

Abstract

Ochratoxin A is an important mycotoxin that can enter the human food chain in cereals, wine, coffee, spices, beer, cocoa, dried fruits, and pork meats. Coffee is one of the most common beverages and, consequently, it has a potential risk factor for human health related to ochratoxin A exposure. In this study, coffee and corresponding byproducts from seven different geographic regions were investigated for ochratoxin A natural occurrence by HPLC-FLD, nutritional characterization, and antioxidant activities by spectrophotometric assay. The research focused on composition changes in coffee during the processing step "from field to cup". Costa Rica and Indian green coffees were the most contaminated samples, with 13 and 11 microg/kg, respectively, while the Ethiopian coffee was the least contaminated, with 3.8 microg/kg of ochratoxin A. The reduction of ochratoxin A contamination during the roasting step was comparable for any samples that were considered under the recommended level of 4 microg/kg. Total dietary fibers ranged from 58.7% for Vietnam and 48.6% for Ivory Coast in green coffees and ranged from 58.6% for Costa Rica to 61.2% for India in roasted coffee. Coffee silverskin byproduct obtained from Ivory Coast was the highest, with 69.2 and 64.2% of insoluble dietary fibers, respectively.

摘要

赭曲霉毒素A是一种重要的霉菌毒素,可通过谷物、葡萄酒、咖啡、香料、啤酒、可可、干果和猪肉进入人类食物链。咖啡是最常见的饮品之一,因此,与接触赭曲霉毒素A相关的人类健康存在潜在风险因素。在本研究中,通过高效液相色谱-荧光检测法(HPLC-FLD)对来自七个不同地理区域的咖啡及其相应副产物中赭曲霉毒素A的天然存在情况进行了调查,并通过分光光度法测定了其营养特性和抗氧化活性。该研究聚焦于咖啡在“从田间到杯中的”加工步骤中的成分变化。哥斯达黎加和印度的生咖啡是污染最严重的样本,赭曲霉毒素A含量分别为13微克/千克和11微克/千克,而埃塞俄比亚咖啡污染最少,赭曲霉毒素A含量为3.8微克/千克。对于任何被认为低于4微克/千克推荐水平的样本,烘焙步骤中赭曲霉毒素A污染的减少情况相当。生咖啡中总膳食纤维含量在越南为58.7%,在象牙海岸为48.6%;烘焙咖啡中总膳食纤维含量在哥斯达黎加为58.6%,在印度为61.2%。从象牙海岸获得的咖啡银皮副产物中不溶性膳食纤维含量最高,分别为69.2%和64.2%。

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