Benson R E, Jones D W, Dodds W J
Am J Vet Res. 1983 Mar;44(3):399-403.
Factors that improved the efficiency, precision, and sensitivity of the electroimmunoassay for canine factor VIII-related antigen were investigated. These included rapid electrophoresis, increased rocket heights, deletion of the gel-washing step, and doubling of the sample capacity per plate. The modified assay was reliable and accurate, could be completed within 3 hours, and permitted twice as many determinations. Other experimental variables that affected the assay were the type of agarose and sample diluent, storage conditions of samples before assay, source of antibody, and use of 2 mM calcium lactate.
研究了提高犬因子VIII相关抗原免疫电泳测定效率、精度和灵敏度的因素。这些因素包括快速电泳、增加火箭峰高度、省略凝胶洗涤步骤以及将每板样品容量加倍。改良后的测定方法可靠且准确，可在3小时内完成，测定次数增加了一倍。影响该测定的其他实验变量包括琼脂糖和样品稀释剂的类型、测定前样品的储存条件、抗体来源以及使用2 mM乳酸钙。
