[利用大鼠子宫进行前列腺素的生物测定]

[Biological assay of prostaglandin using the rat uterus].

作者信息

Takeuchi K, Yamada K, Aizawa Y

出版信息

Nihon Yakurigaku Zasshi. 1975 Oct;71(7):675-82. doi: 10.1254/fpj.71.675.

DOI:10.1254/fpj.71.675

Abstract

Isometric contraction of rat uterus with PGF2alpha was more reliable and stable than when a stomach preparation was used. The method of PG bioassay using rat uterus was as follows: ovariectomized rats were given 10 mug of estrone s.c. 48 hr before the decapitation. Uterine strips were suspended in 2 ml of organ bath containing modified Locke-Ringer solution at 25 degrees C, and the isometric contraction was determined. As little as 4 ng/ml of PGF2alpha was determined quantitatively with this method.

摘要

与使用胃制备物时相比，用前列腺素F2α（PGF2α）进行大鼠子宫等长收缩更为可靠和稳定。使用大鼠子宫进行PG生物测定的方法如下：在断头前48小时，给去卵巢大鼠皮下注射10微克雌酮。将子宫条悬于2毫升含改良洛克-林格溶液的器官浴中，于25℃测定等长收缩。用该方法可定量测定低至4纳克/毫升的PGF2α。