[Biological assay of prostaglandin in rat urine using fundus of rat stomach (author's transl)].

Male Wistar rats, weighing about 180 g, were used. Strips from the fundus of the stomach were prepared from animals deprived of food for 24 hours and fed for 10 minutes before the decapitation. The strips were suspended in 2 ml of organ bath containing Tyrode solution kept at 30 degrees C, and the isometric contraction was determined. Prostaglandins in rat urine were separated by column chromatography and determined by biological assay. No less than 50 pg of PGE2 and 250 pg of PGF2 alpha with good precision could be determined quantitatively using this particular bioassay. Excretion of urinary PGE in the healthy male rat was 29+8+/-1.79 ng/day and PGF was 1.9+/-0.27 ng/day.