Foster F S, Zhang M Y, Zhou Y Q, Liu G, Mehi J, Cherin E, Harasiewicz K A, Starkoski B G, Zan L, Knapik D A, Adamson S L
Dept. of Medical Biophysics, Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Ontario, Canada.
Ultrasound Med Biol. 2002 Sep;28(9):1165-72. doi: 10.1016/s0301-5629(02)00567-7.
We report here on the design and evaluation of the first high-frequency ultrasound (US) imaging system specifically designed for microimaging of the mouse. High-frequency US or US biomicroscopy (UBM) has the advantage of low cost, rapid imaging speed, portability and high resolution. In combination with the ability to provide functional information on blood flow, UBM provides a powerful method for the investigation of development and disease models. The new UBM imaging system is demonstrated for mouse development from day 5.5 of embryogenesis through to the adult mouse. At a frequency of 40 MHz, the resolution voxel of the new mouse scanner measures 57 microm x 57 microm x 40 microm. Duplex Doppler provides blood velocity sensitivity to the mm per s range, consistent with flow in the microcirculation, and can readily detect blood flow in the embryonic mouse heart, aorta, liver and placenta. Noninvasive UBM assessment of development shows striking similarity to invasive atlases of mouse anatomy. The most detailed noninvasive in vivo images of mouse embryonic development achieved using any imaging method are presented.
我们在此报告首个专门为小鼠显微成像设计的高频超声（US）成像系统的设计与评估。高频超声或超声生物显微镜（UBM）具有成本低、成像速度快、便携且分辨率高的优点。结合提供血流功能信息的能力，UBM为研究发育和疾病模型提供了一种强大的方法。新型UBM成像系统展示了从胚胎发育第5.5天到成年小鼠的小鼠发育过程。在40兆赫的频率下，新型小鼠扫描仪的分辨率体素为57微米×57微米×40微米。双功多普勒提供对毫米每秒范围内血流速度的敏感度，与微循环中的血流一致，并且能够轻易检测到胚胎小鼠心脏、主动脉、肝脏和胎盘内的血流。发育的非侵入性UBM评估显示出与小鼠解剖学侵入性图谱的显著相似性。呈现了使用任何成像方法所获得的小鼠胚胎发育最详细的非侵入性体内图像。
