使用脉冲场凝胶电泳检测不同奶牛引进方式的奶牛场之间金黄色葡萄球菌菌株群体的差异。
Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices.
作者信息
Middleton J R, Fox L K, Gay J M, Tyler J W, Besser T E
机构信息
Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO 65211, USA.
出版信息
Epidemiol Infect. 2002 Oct;129(2):387-95. doi: 10.1017/s095026880200746x.
The hypothesis tested was that dairy herds which import cattle for replacement or expansion have a higher prevalence of Staphylococcus aureus mastitis and a greater number of new Staphylococcus aureus strains enter their herds than closed herds. Fifteen commercial dairy herds were divided into four groups based on cattle importation practices. Composite foremilk samples were collected at 4-monthly intervals for 1 year from all lactating cattle. Additionally, foremilk samples were collected from cattle at parturition and skin swabs were taken from the udder of primiparous heifers. All samples were cultured for Staphylococcus aureus and isolates were strain-typed using pulsed-field gel electrophoresis. Herds that purchased replacement heifers had a higher prevalence of Staphylococcus aureus mastitis than herds that purchased lactating cattle for expansion (P = 0.02). Herds that purchased replacement heifers had more total strains of Staphylococcus aureus (P = 0.01) and more new strains (P = 0.04) enter the herd than closed herds.
所检验的假设是，为补充或扩大牛群而引进牛只的奶牛场，金黄色葡萄球菌性乳腺炎的患病率较高，且与封闭牛群相比，有更多新的金黄色葡萄球菌菌株进入其牛群。根据牛只引进做法，将15个商业奶牛场分为四组。在1年的时间里，每隔4个月从所有泌乳牛采集混合前乳样本。此外，在分娩时从牛只采集前乳样本，并从初产小母牛的乳房采集皮肤拭子。所有样本均培养金黄色葡萄球菌，并使用脉冲场凝胶电泳对分离株进行菌株分型。购买后备小母牛的牛场，金黄色葡萄球菌性乳腺炎的患病率高于为扩大牛群而购买泌乳牛的牛场（P = 0.02）。与封闭牛群相比，购买后备小母牛的牛场有更多的金黄色葡萄球菌总菌株（P = 0.01）和更多新菌株（P = 0.04）进入牛群。
Zotero 插件