AarI是一种来自金色节杆菌SS2 - 322的限制性内切酶，它识别新的非回文序列5'-CACCTGC(N)4/8-3'。
AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3'.
作者信息
Grigaite R, Maneliene Z, Janulaitis A
机构信息
Fermentas UAB and. Institute of Biotechnology, Graiciūno 8, 2028 Vilnius, Lithuania.
出版信息
Nucleic Acids Res. 2002 Nov 1;30(21):e123. doi: 10.1093/nar/gnf122.
Abstract
A new type II restriction endonuclease AarI has been isolated from Arthrobacter aurescens SS2-322. AarI recognizes the non-palindromic heptanucleotide sequence 5'-CACCTGC(N)4/8-3' and makes a staggered cut at the fourth and eighth bases downstream of the target duplex producing a four base 5'-protruding end. AarI activity is stimulated by oligodeoxyribonucleotide duplexes containing an enzyme-specific recognition sequence.
摘要
一种新型II型限制性内切酶AarI已从金色节杆菌SS2-322中分离出来。AarI识别非回文七核苷酸序列5'-CACCTGC(N)4/8-3'，并在靶双链下游的第四和第八个碱基处进行交错切割，产生一个四碱基的5'-突出末端。含有酶特异性识别序列的寡脱氧核糖核苷酸双链体可刺激AarI的活性。
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