一种新型链霉菌限制性内切酶Sse1825I，切割位点为5'-GG/GWCCC-3'。

A novel Streptomyces restriction endonuclease, Sse1825I, cleaving at 5'-GG/GWCCC-3'.

作者信息

Nomura Y, Ishino Y, Kimizuka F, Kato I

机构信息

Biotechnology research Laboratories, Takara Shuzo Co., Ltd. Otsu, Shiga, Japan.

出版信息

Gene. 1995 May 19;157(1-2):323-4. doi: 10.1016/0378-1119(95)00119-q.

DOI:10.1016/0378-1119(95)00119-q

PMID:7607520

Abstract

We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence [sequence: see text] (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence. This Sse1825I enzyme cleaves phage lambda DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, ColE1, pUC18 and pUC19, and replicative forms of M13mp18 and M13mp19, and phiX174 DNAs.

摘要

我们从一种链霉菌属菌株中分离并鉴定出一种新型II类限制性内切酶，它识别回文七核苷酸序列[序列：见正文]（其中W = A或T），并在该序列中的第二个G后切割双链DNA。这种Sse1825I酶在一个位点切割噬菌体λDNA，在八个位点切割2型腺病毒DNA，但不切割pBR322、SV40、ColE1、pUC18和pUC19，以及M13mp18和M13mp19的复制形式，和phiX174 DNA。