Nomura Y, Ishino Y, Kimizuka F, Kato I
Biotechnology research Laboratories, Takara Shuzo Co., Ltd. Otsu, Shiga, Japan.
Gene. 1995 May 19;157(1-2):323-4. doi: 10.1016/0378-1119(95)00119-q.
We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence [sequence: see text] (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence. This Sse1825I enzyme cleaves phage lambda DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, ColE1, pUC18 and pUC19, and replicative forms of M13mp18 and M13mp19, and phiX174 DNAs.
我们从一种链霉菌属菌株中分离并鉴定出一种新型II类限制性内切酶，它识别回文七核苷酸序列[序列：见正文]（其中W = A或T），并在该序列中的第二个G后切割双链DNA。这种Sse1825I酶在一个位点切割噬菌体λDNA，在八个位点切割2型腺病毒DNA，但不切割pBR322、SV40、ColE1、pUC18和pUC19，以及M13mp18和M13mp19的复制形式，和phiX174 DNA。
