Knobloch Johannes K-M, Horstkotte Matthias A, Rohde Holger, Mack Dietrich
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, Germany.
Med Microbiol Immunol. 2002 Oct;191(2):101-6. doi: 10.1007/s00430-002-0124-3. Epub 2002 Jun 29.
The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.
金黄色葡萄球菌的icaADBC基因位点及其胞间多糖黏附素（PIA/PNSG）最近已被确定，但在体外很少检测到生物膜形成。在本研究中，我们评估了组织培养板（TCP）检测法、试管试验以及刚果红琼脂，使用两种基本培养基胰蛋白酶大豆肉汤（TSB）和脑心浸液（BHI）肉汤，并添加不同的糖类补充剂，以检测128株ica阳性金黄色葡萄球菌分离株中的生物膜形成情况。在金黄色葡萄球菌菌株中，57.1%在TCP试验的优化条件下表现出生物膜阳性表型。对于强生物膜产生菌株，试管试验与TCP试验相关性良好，而弱产生菌株与生物膜阴性菌株无法可靠区分。在刚果红琼脂上进行筛选时，只有3.8%的结果与TCP试验和试管试验有强相关性，因此不建议用于研究金黄色葡萄球菌中的生物膜形成。
