Pietruczuk-Padzik Anna, Stefańska Joanna, Semczuk Katarzyna, Dzierzanowska Danuta, Tyski Stefan
Zakład Mikrobiologii Farmaceutycznej, Warszawski Uniwersytet Medyczny.
Med Dosw Mikrobiol. 2010;62(1):1-8.
The purpose of this study was to evaluate two screening methods for detection of biofilm formation by eighty clinical Staphylococcus aureus isolates from patients with cystic fibrosis, and evaluation of biofilm production on the polystyrene 96-well tissue culture plates, depending on media applied. All clinical strains were incubated in three different media: Luria-Bertani broth (LB), tryptic soy broth supplemented with 2% glucose (TSBglu) and brain heart infusion (BHI). Biofilm production was screened by staining with crystal violet (CV) or with 2,3,5-triphenyltetrazolium chloride (TTC). Both CV and TTC assays showed, that all analyzed isolates created biofilm, in all tested media, however with different intensity. In conclusion, the CV method was found to be more sensitive than the TTC method, when we need information about whole mass of biofilm. The most optimal medium for the biofilm culture was LB medium.
本研究的目的是评估两种用于检测来自囊性纤维化患者的80株临床金黄色葡萄球菌分离株生物膜形成的筛选方法，并根据所使用的培养基评估在聚苯乙烯96孔组织培养板上生物膜的产生情况。所有临床菌株均在三种不同的培养基中培养：Luria-Bertani肉汤（LB）、补充2%葡萄糖的胰蛋白胨大豆肉汤（TSBglu）和脑心浸液（BHI）。通过用结晶紫（CV）或2,3,5-三苯基氯化四氮唑（TTC）染色来筛选生物膜的产生。CV和TTC检测均显示，所有分析的分离株在所有测试培养基中均形成了生物膜，但强度不同。总之，当我们需要了解生物膜的整体质量信息时，发现CV方法比TTC方法更敏感。生物膜培养的最佳培养基是LB培养基。
