[茶多酚和茶色素对大鼠肝癌前病变细胞周期调节因子的影响]
[Effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions].
作者信息
Jia Xudong, Han Chi, Chen Junshi
机构信息
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2002 Jul;36(4):225-8.
OBJECTIVES
This study is to investigate the effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions.
METHODS
The modified Solt-Farber precancerous liver rat model was used. Rats were given water, tea polypheol solution (0.1%) or tea pigment solution (0.1%) throughout the whole experiment (56 days). Cyclin D1, P21(WAF1/CIP1), GADD45 and PCNA protein expression were detected by Western blotting and the RT-PCR method was applied to study the expression of Cdk4.
RESULTS
Cyclin D1, Cdk4 and PCNA expressions were significantly inhibited, and the expression of P21(WAF1/CIP1) and GADD45 were significantly induced by tea polyphenols and tea pigments treatments.
CONCLUSION
Tea polyphenols and tea pigments induced cell cycle arrest and inhibited cell proliferation by regulating cell cycle regulators.
目的
本研究旨在探讨茶多酚和茶色素对大鼠肝脏癌前病变中细胞周期调节因子的影响。
方法
采用改良的索尔-法伯肝癌前大鼠模型。在整个实验（56天）过程中，给大鼠分别饮用蒸馏水、茶多酚溶液（0.1%）或茶色素溶液（0.1%）。通过蛋白质免疫印迹法检测细胞周期蛋白D1、P21（WAF1/CIP1）、生长停滞和DNA损伤诱导蛋白45（GADD45）以及增殖细胞核抗原（PCNA）的蛋白表达，并应用逆转录聚合酶链反应（RT-PCR）法研究细胞周期蛋白依赖性激酶4（Cdk4）的表达。
结果
茶多酚和茶色素处理可显著抑制细胞周期蛋白D1、Cdk4和PCNA的表达，并显著诱导P21（WAF1/CIP1）和GADD45的表达。
结论
茶多酚和茶色素通过调节细胞周期调节因子诱导细胞周期停滞并抑制细胞增殖。
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