[结直肠癌化学预防研究中的生物标志物]

[Biomarkers in the studies on chemoprevention of colorectal cancer].

作者信息

Jia X, Han C

机构信息

Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.

出版信息

Wei Sheng Yan Jiu. 2000 Mar 30;29(2):109-11.

PMID:12725091

Abstract

The present study was to investigate the chemopreventive effects of tea on colorectal cancer with a series of biomarkers. The results showed that the number of silver-stained nucleolar organizer dots per nucleus(AgNORs), labeling index(LI) of proliferating cell nuclear antigen(PCNA) of intestinal mucosa, and the expression of ras-p21 protein were significantly reduced in the tea-treated groups(P < 0.01) as compared with the positive control group. Furthermore, tea and tea pigments inhibited the expression of Bcl-2 protein and induced the expression of Bax protein(P < 0.05 or P < 0.01). The study provided evidence supporting that PCNA-LI, AgNORs, Bcl-2, Bax and ras-p21 protein could be used as effective biomarkers for colorectal carcinogenesis in human chemopreventive trials.

摘要

本研究旨在通过一系列生物标志物探讨茶对结直肠癌的化学预防作用。结果显示，与阳性对照组相比，茶处理组中每个细胞核的银染核仁组织区点数（AgNORs）、肠黏膜增殖细胞核抗原（PCNA）的标记指数（LI）以及ras-p21蛋白的表达均显著降低（P < 0.01）。此外，茶和茶色素抑制了Bcl-2蛋白的表达并诱导了Bax蛋白的表达（P < 0.05或P < 0.01）。该研究提供了证据支持PCNA-LI、AgNORs、Bcl-2、Bax和ras-p21蛋白可作为人类化学预防试验中结直肠癌发生的有效生物标志物。