[硒和氟对人肝细胞凋亡及脂质过氧化的影响]

[Effects of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes].

作者信息

Wang Aiguo, Xia Tao, Ran Peng, Bai Yun, Yang Kedi, Chen Xuemin

机构信息

Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2002 Jul;36(4):235-8.

PMID:12411202

Abstract

OBJECTIVE

To study the influence of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes in vitro.

METHODS

The apoptosis, cell cycle, GSH content and lipid peroxides (LPO) level in human hepatocytes, LPO level and LDH, AST and ALT activity in cell culture supernatants were investigated after hepatocytes were incubated with selenium and/or fluoride for around 12 hours periods in vitro.

RESULTS

The percentage of hepatocyte apoptosis bodies (15.557 +/- 2.056)%, the number of cells in S phase (4.823 +/- 0.454)% and LPO level in liver tissue and supernatant [(2.884 +/- 0.589) and (3.547 +/- 0.561) nmol/L MDA/mg.prot, respectively], AST and LDH activity in supernatants (91.1 +/- 36.4 and 140.4 +/- 7.6 U/L, respectively) in the fluoride treated group was higher than the control group [(10.313 +/- 1.023)%, (3.253 +/- 0.743)%, (1.473 +/- 0.401) nmol/L MDA/mg.prot, (1.694 +/- 0.443) nmol/L MDA/mg.prot, (54.5 +/- 3.2) U/L and (126.4 +/- 2.6) U/L, respectively], The GSH content in live tissue [(4.225 +/- 0.781) microgram/mg.prot] is lower than control group [(7.595 +/- 1.042) microgram/mg.prot]. Selenium treatment reduced these kinds of toxicity of fluoride through raising GSH content, reducing LPO level, LDH and AST activity and percentage of apoptosis bodies.

CONCLUSIONS

Selenium can antagonist apoptosis and lipid peroxidation of hepatocytes induced by fluoride.

摘要

目的

研究硒和氟对体外培养的人肝细胞凋亡及脂质过氧化的影响。

方法

体外将人肝细胞与硒和/或氟孵育约12小时后，检测肝细胞的凋亡、细胞周期、谷胱甘肽（GSH）含量和脂质过氧化物（LPO）水平，以及细胞培养上清液中的LPO水平、乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性。

结果

氟处理组肝细胞凋亡小体百分比（15.557±2.056）%、S期细胞数（4.823±0.454）%、肝组织和上清液中LPO水平[分别为（2.884±0.589）和（3.547±0.561）nmol/L丙二醛（MDA）/mg蛋白]、上清液中AST和LDH活性[分别为（91.1±36.4）和（140.4±7.6）U/L]均高于对照组[分别为（10.313±1.023）%、（3.253±0.743）%、（1.473±0.401）nmol/L MDA/mg蛋白、（1.694±0.443）nmol/L MDA/mg蛋白、（54.5±3.2）U/L和（126.4±2.6）U/L]，肝组织中GSH含量[（4.225±0.781）μg/mg蛋白]低于对照组[（7.595±1.042）μg/mg蛋白]。硒处理通过提高GSH含量、降低LPO水平、LDH和AST活性以及凋亡小体百分比，减轻了氟的这些毒性作用。