Fabrizio K A, Sharma R R, Demirci A, Cutter C N
Department of Food Science, The Pennsylvania State University, University Park 16802, USA.
Poult Sci. 2002 Oct;81(10):1598-605. doi: 10.1093/ps/81.10.1598.
Foodborne pathogens in cell suspensions or attached to surfaces can be reduced by electrolyzed oxidizing (EO) water; however, the use of EO water against pathogens associated with poultry has not been explored. In this study, acidic EO water [EO-A; pH 2.6, chlorine (CL) 20 to 50 ppm, and oxidation-reduction potential (ORP) of 1,150 mV], basic EO water (EO-B; pH 11.6, ORP of -795 mV), CL, ozonated water (OZ), acetic acid (AA), or trisodium phosphate (TSP) was applied to broiler carcasses inoculated with Salmonella Typhimurium (ST) and submerged (4 C, 45 min), spray-washed (85 psi, 25 C, 15 s), or subjected to multiple interventions (EO-B spray, immersed in EO-A; AA or TSP spray, immersed in CL). Remaining bacterial populations were determined and compared at Day 0 and 7 of aerobic, refrigerated storage. At Day 0, submersion in TSP and AA reduced ST 1.41 log10, whereas EO-A water reduced ST approximately 0.86 log10. After 7 d of storage, EO-A water, OZ, TSP, and AA reduced ST, with detection only after selective enrichment. Spray-washing treatments with any of the compounds did not reduce ST at Day 0. After 7 d of storage, TSP, AA, and EO-A water reduced ST 2.17, 2.31, and 1.06 log10, respectively. ST was reduced 2.11 log10 immediately following the multiple interventions, 3.81 log10 after 7 d of storage. Although effective against ST, TSP and AA are costly and adversely affect the environment. This study demonstrates that EO water can reduce ST on poultry surfaces following extended refrigerated storage.
细胞悬液中或附着于表面的食源性病原体可通过电解氧化（EO）水减少；然而，EO水针对与家禽相关病原体的应用尚未得到探索。在本研究中，将酸性EO水[EO-A；pH 2.6，氯（CL）20至50 ppm，氧化还原电位（ORP）为1150 mV]、碱性EO水（EO-B；pH 11.6，ORP为-795 mV）、CL、臭氧水（OZ）、乙酸（AA）或磷酸三钠（TSP）应用于接种了鼠伤寒沙门氏菌（ST）的肉鸡胴体，并进行浸没处理（4℃，45分钟）、喷雾清洗（85 psi，25℃，15秒）或进行多次干预（EO-B喷雾，浸入EO-A中；AA或TSP喷雾，浸入CL中）。在需氧、冷藏储存的第0天和第7天测定并比较剩余细菌数量。在第0天，浸没于TSP和AA中使ST减少1.41 log10，而EO-A水使ST减少约0.86 log10。储存7天后，EO-A水、OZ、TSP和AA使ST减少，仅在选择性富集后才检测到。在第0天，用任何一种化合物进行喷雾清洗处理均未减少ST。储存7天后，TSP、AA和EO-A水分别使ST减少2.17、2.31和1.06 log10。多次干预后ST立即减少2.11 log10，储存7天后减少3.81 log10。尽管TSP和AA对ST有效，但成本高昂且对环境有不利影响。本研究表明，延长冷藏储存后，EO水可减少家禽表面的ST。
