一种能够引发大幅pH值跃升的新型笼蔽质子的特性研究。
Characterization of a new caged proton capable of inducing large pH jumps.
作者信息
Barth Andreas, Corrie John E T
机构信息
Institut für Biophysik, Johann Wolfgang Goethe-Universität, D-60590 Frankfurt am Main, Germany.
出版信息
Biophys J. 2002 Nov;83(5):2864-71. doi: 10.1016/S0006-3495(02)75295-8.
Abstract
A new caged proton, 1-(2-nitrophenyl)ethyl sulfate (caged sulfate), is characterized by infrared spectroscopy and compared with a known caged, proton 2-hydroxyphenyl 1-(2-nitrophenyl)ethyl phosphate (caged HPP). In contrast to caged HPP, caged sulfate can induce large pH jumps and protonate groups that have pK values as low as 2.2. The photolysis mechanism of caged sulfate is analogous to that of P(3)-[1-(2-nitrophenyl)ethyl] ATP (caged ATP), and the photolysis efficiency is similar. The utility of this new caged compound for biological studies was demonstrated by its ability to drive the acid-induced conformational change of metmyoglobin. This transition from the native conformation to a partially unfolded form takes place near pH 4 and was monitored by near-UV absorption spectroscopy.
摘要
一种新型笼形质子，1-(2-硝基苯基)乙基硫酸盐（笼形硫酸盐），通过红外光谱进行了表征，并与已知的笼形质子2-羟基苯基1-(2-硝基苯基)乙基磷酸盐（笼形HPP）进行了比较。与笼形HPP不同，笼形硫酸盐可诱导大幅pH跃变，并使pK值低至2.2的基团质子化。笼形硫酸盐的光解机制与P(3)-[1-(2-硝基苯基)乙基]ATP（笼形ATP）类似，且光解效率相近。这种新型笼形化合物在生物学研究中的效用通过其驱动高铁肌红蛋白酸诱导构象变化的能力得到了证明。这种从天然构象到部分展开形式的转变发生在pH 4附近，并通过近紫外吸收光谱进行了监测。
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