用活重组牛呼吸道合胞病毒（BRSV）和缺乏包膜糖蛋白G的重组BRSV进行黏膜免疫可抵御野生型BRSV的攻击。
Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV.
作者信息
Schmidt Ulrike, Beyer Jörg, Polster Ulf, Gershwin Laurel J, Buchholz Ursula J
机构信息
Institute of Molecular Biology, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany.
出版信息
J Virol. 2002 Dec;76(23):12355-9. doi: 10.1128/jvi.76.23.12355-12359.2002.
Abstract
Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSVDeltaG) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSVDeltaG led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSVDeltaG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV.
摘要
对重组牛呼吸道合胞病毒（rBRSV）和缺失G基因的rBRSV缺失突变体（rBRSVDeltaG）作为减毒活BRSV疫苗，在犊牛中进行了复制能力、减毒效果和保护效力方面的特性研究。两种重组病毒均安全，并诱导产生了针对BRSV攻击感染的保护作用。rBRSV在上呼吸道中高效复制。用rBRSVDeltaG进行鼻内免疫导致感染，但未引起黏膜病毒复制。rBRSV和rBRSVDeltaG均可诱导中和抗体。因此，BRSV附着糖蛋白G在犊牛接种疫苗预防BRSV方面似乎并非必需。
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