Oriani D S, Sagardoy M A
Facultad de Ciencias Veterinarias, Universidad Nacional de La Pampa, 6360 General Pico, Argentina.
Rev Argent Microbiol. 2002 Jul-Sep;34(3):132-7.
The presence of nontuberculous mycobacteria (NTM) was investigated in forty soil samples belonging to the four physiographic regions (Eastern, Central, Southern and Western) that constitute La Pampa province. The presence of NTM in 67.5% of these soil samples was determined. The density of mycobacteria ranged 25-4,500 mycobacteria g-1 dry soil (mean = 516 CFU g-1). Significant differences were found in relation to both the investigated regions (p < 0.01) and the soil pH (r = 0.44*) (P = 0.02). The mycobacteria represented less than 0.00001% of the total aerobic bacteria found in the soils. Twenty-seven isolated mycobacteria were classified according to the culture, biochemical, enzymatic characteristics and antibiotic sensitivity. Mycobacterium fortitium was the dominant mycobacterium and was detected in 63% of the positive soils. This species showed ability for living in sandy to sandy loam soils, within a wide pH range (6.5-9.7) and organic matter (4.15-83.63 g kg-1). Two other species were M. phlei (range = 50-4,500 CFU g-1) and M. kansasii (range = 50-500 CFU g-1).
在构成拉潘帕省的四个自然地理区域（东部、中部、南部和西部）的40个土壤样本中，对非结核分枝杆菌（NTM）的存在情况进行了调查。结果确定，这些土壤样本中有67.5%存在NTM。分枝杆菌密度在25 - 4500个分枝杆菌/克干土之间（平均 = 516 CFU/克）。在调查区域（p < 0.01）和土壤pH值（r = 0.44*）（P = 0.02）方面均发现了显著差异。分枝杆菌在土壤中发现的总需氧菌中所占比例不到0.00001%。根据培养、生化、酶学特征和抗生素敏感性对27株分离的分枝杆菌进行了分类。坚强分枝杆菌是优势分枝杆菌，在63%的阳性土壤中被检测到。该物种显示出能够生活在砂土至砂壤土中，在较宽的pH范围（6.5 - 9.7）和有机质含量（4.15 - 83.63克/千克）内生存。另外两个物种是草分枝杆菌（范围 = 50 - 4,500 CFU/克）和堪萨斯分枝杆菌（范围 = 50 - 500 CFU/克）。
