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分枝杆菌病血清学诊断中预吸附步骤的替代物：偶然分枝杆菌。

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum.

机构信息

Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense , Niterói, RJ , Brasil.

出版信息

Braz J Microbiol. 2008 Jul;39(3):511-3. doi: 10.1590/S1517-838220080003000019. Epub 2008 Sep 1.

DOI:10.1590/S1517-838220080003000019
PMID:24031256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3768432/
Abstract

ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.

摘要

ELISAs 用于副结核病采用分枝杆菌 phlei 的预吸附步骤来减少非特异性反应，因为 M. fortuitum 是最常见的环境分枝杆菌之一，本初步研究的目的是评估其作为副结核病 ELISA 中预吸附的替代物的用途。结果表明，M. fortuitum 可以替代或与分枝杆菌 phlei 联合使用，结果相当（κ > 0.8），与使用分枝杆菌 phlei 作为预吸附抗原的常规 ELISA 相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/3768432/0b970e84811e/bjm-39-511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/3768432/0b970e84811e/bjm-39-511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/3768432/0b970e84811e/bjm-39-511-g001.jpg

相似文献

1
An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum.分枝杆菌病血清学诊断中预吸附步骤的替代物：偶然分枝杆菌。
Braz J Microbiol. 2008 Jul;39(3):511-3. doi: 10.1590/S1517-838220080003000019. Epub 2008 Sep 1.
2
[Asparagine metabolism in mycobacteria. II. -- Asparagine hydrolysis and aspartohydroxamic acid formation and hydrolysis catalysed by M. fortuitum, M. phlei and BCG asparaginases (author's transl)].分枝杆菌中的天冬酰胺代谢。II. —— 偶然分枝杆菌、草分枝杆菌和卡介苗天冬酰胺酶催化的天冬酰胺水解、天冬氨酸异羟肟酸形成及水解（作者译）
Ann Microbiol (Paris). 1975 Feb-Mar;126(2):151-60.
3
Contribution of environmental mycobacteria to false-positive serum ELISA results for paratuberculosis.环境分枝杆菌对副结核病血清ELISA检测结果假阳性的影响
J Am Vet Med Assoc. 2007 Mar 15;230(6):896-901. doi: 10.2460/javma.230.6.896.
4
Isolation of specific peptides from Mycobacterium paratuberculosis protoplasm and their use in an enzyme-linked immunosorbent assay for the detection of paratuberculosis (Johne's disease) in cattle.从副结核分枝杆菌原生质体中分离特定肽段及其在酶联免疫吸附测定法中用于检测牛副结核病（约内氏病）的应用。
Am J Vet Res. 1983 Dec;44(12):2229-36.
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Analysis by ELISA and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei.在用草分枝杆菌吸收血清后，对感染副结核分枝杆菌的牛的抗体反应性进行酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析。
Res Vet Sci. 1987 Mar;42(2):140-4.
6
Comparison of the 23S ribosomal RNA genes and the spacer region between the 16S and 23S rRNA genes of the closely related Mycobacterium avium and Mycobacterium paratuberculosis and the fast-growing Mycobacterium phlei.密切相关的鸟分枝杆菌和副结核分枝杆菌以及快速生长的草分枝杆菌的23S核糖体RNA基因和16S与23S rRNA基因之间间隔区的比较。
Microbiology (Reading). 1994 May;140 ( Pt 5):1103-8. doi: 10.1099/13500872-140-5-1103.
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Antibody reactivities of Mycobacterium paratuberculosis infected sheep as analyzed by enzyme-linked immunosorbent assay and western blotting.通过酶联免疫吸附测定和蛋白质印迹分析副结核分枝杆菌感染绵羊的抗体反应性。
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):145-8. doi: 10.1016/0378-1097(91)90148-4.
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The specificity of the fluorescent antibody test using the sera of rabbits inoculated with strains of myocobacteria.使用接种了分枝杆菌菌株的兔血清进行荧光抗体试验的特异性。
Res Vet Sci. 1976 Jan;20(1):1-5.
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[Comparative study of mycobactin-dependent strains of mycobacteria isolated from the wood-pigeon with Mycobacterium avium and M. paratuberculosis: study of biological and antigenic characteristics].[从林鸽分离的分枝杆菌的铁载体依赖性菌株与鸟分枝杆菌和副结核分枝杆菌的比较研究：生物学和抗原特性研究]
Ann Microbiol (Paris). 1982 Sep-Oct;133(2):291-302.
10
Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples.开发一种针对独特多拷贝元件ISMap02的巢式PCR方法，用于检测粪便样本中的副结核分枝杆菌。
J Clin Microbiol. 2005 Sep;43(9):4744-50. doi: 10.1128/JCM.43.9.4744-4750.2005.

本文引用的文献

1
Contribution of environmental mycobacteria to false-positive serum ELISA results for paratuberculosis.环境分枝杆菌对副结核病血清ELISA检测结果假阳性的影响
J Am Vet Med Assoc. 2007 Mar 15;230(6):896-901. doi: 10.2460/javma.230.6.896.
2
Isolation and Identification of Environmental Mycobacteria in the Mycobacterium bovis BCG Trial Area of South India.印度南部牛分枝杆菌卡介苗试验区环境分枝杆菌的分离与鉴定。
Appl Environ Microbiol. 1994 Jun;60(6):2180-3. doi: 10.1128/aem.60.6.2180-2183.1994.
3
Evaluation of five antibody detection tests for diagnosis of bovine paratuberculosis.
用于诊断牛副结核病的五种抗体检测试验的评估
Clin Diagn Lab Immunol. 2005 Jun;12(6):685-92. doi: 10.1128/CDLI.12.6.685-692.2005.
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Model for immune responses to Mycobacterium avium subspecies paratuberculosis in cattle.牛对副结核分枝杆菌鸟型亚种免疫反应的模型
Infect Immun. 2004 Jun;72(6):3089-96. doi: 10.1128/IAI.72.6.3089-3096.2004.
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Isolation and identification of mycobacteria from livestock specimens and milk obtained in Brazil.从巴西采集的家畜标本和牛奶中分离和鉴定分枝杆菌。
Mem Inst Oswaldo Cruz. 2003 Apr;98(3):319-23. doi: 10.1590/s0074-02762003000300005. Epub 2003 Jul 18.
6
Nontuberculous mycobacteria in soils of La Pampa province (Argentina).阿根廷潘帕斯省土壤中的非结核分枝杆菌。
Rev Argent Microbiol. 2002 Jul-Sep;34(3):132-7.
7
Genome scale comparison of Mycobacterium avium subsp. paratuberculosis with Mycobacterium avium subsp. avium reveals potential diagnostic sequences.鸟分枝杆菌副结核亚种与鸟分枝杆菌亚种的全基因组规模比较揭示了潜在的诊断序列。
J Clin Microbiol. 2002 Apr;40(4):1303-10. doi: 10.1128/JCM.40.4.1303-1310.2002.
8
Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis.烷基过氧化氢还原酶C和D是副结核分枝杆菌亚种组成性表达的主要抗原。
Infect Immun. 2000 Feb;68(2):801-8. doi: 10.1128/IAI.68.2.801-808.2000.
9
Mycobacteria. General culture methodology and safety considerations.分枝杆菌。一般培养方法及安全注意事项。
Methods Mol Biol. 1998;101:15-30. doi: 10.1385/0-89603-471-2:15.
10
Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows.一种草分枝杆菌吸附血清酶联免疫吸附测定法诊断奶牛副结核病的诊断准确性
J Clin Microbiol. 1992 Mar;30(3):613-8. doi: 10.1128/jcm.30.3.613-618.1992.