Tissue cultures from adult human postmortem subcortical brain areas.
作者信息
Verwer R W H, Dubelaar E J G, Hermens W T J M C, Swaab D F
机构信息
Graduate School Neurosciences Amsterdam, Netherlands Institute for Brain Research, Amsterdam, The Netherlands.
出版信息
J Cell Mol Med. 2002 Jul-Sep;6(3):429-32. doi: 10.1111/j.1582-4934.2002.tb00522.x.
Abstract
Animal models used to study human aging and neurodegeneration do not display all symptoms of these processes as they are found in humans. Recently, we have shown that many cells in neocortical slices from adult human postmortem brain may survive for extensive periods in vitro. Such cultures may enable us to study age and disease related processes directly in human brain tissue. Here, we present observations on subcortical brain tissue.
摘要
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本文引用的文献
1
Post-mortem brain tissue cultures from elderly control subjects and patients with a neurodegenerative disease.来自老年对照受试者和神经退行性疾病患者的尸检脑组织培养物。
Exp Gerontol. 2003 Jan-Feb;38(1-2):167-72. doi: 10.1016/s0531-5565(02)00154-7.
2
Life after death?
Neurology. 2002 Nov 12;59(9):1355. doi: 10.1212/01.wnl.0000032758.08215.d1.
3
The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics.阿尔茨海默病的淀粉样蛋白假说：治疗之路上的进展与问题
Science. 2002 Jul 19;297(5580):353-6. doi: 10.1126/science.1072994.
4
Animal models of neurological deficits: how relevant is the rat?神经功能缺损的动物模型：大鼠的相关性如何？
Nat Rev Neurosci. 2002 Jul;3(7):574-9. doi: 10.1038/nrn877.
5
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FASEB J. 2002 Jan;16(1):54-60. doi: 10.1096/fj.01-0504com.
6
Purification of recombinant adeno-associated virus by iodixanol gradient ultracentrifugation allows rapid and reproducible preparation of vector stocks for gene transfer in the nervous system.通过碘克沙醇梯度超速离心法纯化重组腺相关病毒，能够快速且可重复地制备用于神经系统基因转移的载体储备液。
Hum Gene Ther. 1999 Jul 20;10(11):1885-91. doi: 10.1089/10430349950017563.
7
A procedure for culturing rat neocortex explants in a serum-free nutrient medium.
J Neurosci Methods. 1988 Feb;23(1):75-83. doi: 10.1016/0165-0270(88)90025-8.
8
Neuron numbers and sizes in aging brain: comparisons of human, monkey, and rodent data.衰老大脑中的神经元数量和大小：人类、猴子和啮齿动物数据的比较。
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