转录激活区域靶向一种细胞周期蛋白依赖性激酶。
Transcriptional activating regions target a cyclin-dependent kinase.
作者信息
Ansari Aseem Z, Koh Sang Seok, Zaman Zafar, Bongards Christine, Lehming Norbert, Young Richard A, Ptashne Mark
机构信息
Program in Molecular Biology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14706-9. doi: 10.1073/pnas.232573899. Epub 2002 Nov 4.
Abstract
Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidic transcriptional activating regions contact SRB10 as assayed both in vivo and in vitro. The interaction evidently positions each activator, as it activates transcription, so that it gets phosphorylated by SRB10, and thus a common mechanism targets disparate substrates to the kinase.
摘要
几种酵母激活因子被SRB10磷酸化，SRB10是一种与转录机制相关的细胞周期蛋白依赖性激酶。在每种情况下，磷酸化位点都位于激活区域之外，并且这种修饰在不同情况下具有不同的生理后果。我们在此表明，某些酸性转录激活区域在体内和体外测定中都与SRB10接触。这种相互作用显然在激活转录时定位每个激活因子，使其被SRB10磷酸化，因此一种共同机制将不同的底物靶向该激酶。
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