用于精确测量蛋白质中一键15N-1H(N)、15N-13C'以及二键13C'-1H(N)剩余偶极耦合的灵敏度增强型E.COSY类HSQC实验。

Sensitivity-enhanced E.COSY-type HSQC experiments for accurate measurements of one-bond 15N-1H(N) and 15N-13C' and two-bond 13C'-1H(N) residual dipolar couplings in proteins.

作者信息

Ding Keyang, Gronenborn Angela M

机构信息

Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

J Magn Reson. 2002 Sep-Oct;158(1-2):173-7. doi: 10.1016/s1090-7807(02)00024-1.

DOI:10.1016/s1090-7807(02)00024-1

PMID:12419684

Abstract

Novel E.COSY-type HSQC experiments are presented for the accurate measurement of one-bond 15N-1H(N) and 15N-13C(') and two-bond 13C(')-1H(N) residual dipolar couplings in proteins. Compared with existing experiments, the (delta,J)-E.COSY experiments described here are composed of fewer pulses and the resulting spectra exhibit 1.4 times the sensitivity of coupled HSQC spectra. Since residual dipolar couplings play increasingly important roles in structural NMR, the proposed methods should find wide spread application for structure determination of proteins and other biological macromolecules.

摘要

本文提出了新型E.COSY型HSQC实验，用于精确测量蛋白质中一键15N-1H(N)和15N-13C(')以及二键13C(')-1H(N)的剩余偶极耦合。与现有实验相比，这里描述的(delta,J)-E.COSY实验由更少的脉冲组成，所得光谱的灵敏度是耦合HSQC光谱的1.4倍。由于剩余偶极耦合在结构核磁共振中发挥着越来越重要的作用，所提出的方法应能广泛应用于蛋白质和其他生物大分子的结构测定。