Ding Keyang, Gronenborn Angela M
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Am Chem Soc. 2003 Sep 24;125(38):11504-5. doi: 10.1021/ja035954e.
Using the echo-anti-echo manipulation, the 15N-1HN cross-peaks split in the E.COSY spectrum by the 13CO couplings are separated into different, distinct regions in the HSQC spectrum. From this novel E.COSY 15N-1HN HSQC spectrum, the small one-bond 15N-13C' and two-bond 1HN-13C' residual dipolar couplings can be extracted easily and accurately. These dipolar couplings provide a set of important long-range constraints for protein structure determination.
通过回波-反回波操作，在E.COSY谱中因13CO耦合而分裂的15N-1HN交叉峰在HSQC谱中被分离到不同的、清晰的区域。从这个新颖的E.COSY 15N-1HN HSQC谱中，可以轻松且准确地提取出小的一键15N-13C'和二键1HN-13C'残余偶极耦合。这些偶极耦合为蛋白质结构测定提供了一组重要的长程限制条件。
