甜菊糖苷对12-O-十四烷酰佛波醇-13-乙酸酯在小鼠皮肤两阶段致癌过程中促进肿瘤作用的抑制效果。

Inhibitory effect of stevioside on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin.

作者信息

Yasukawa Ken, Kitanaka Susumu, Seo Shujiro

机构信息

College of Pharmacy, Nihon University, Chiba, Japan.

出版信息

Biol Pharm Bull. 2002 Nov;25(11):1488-90. doi: 10.1248/bpb.25.1488.

DOI:10.1248/bpb.25.1488

PMID:12419967

Abstract

Four steviol (ent-kaurene-type diterpenoid) glycosides, stevioside, rebaudiosides A and C, and dulcoside A, have been isolated from Stevia rebaudiana BERTONI. These compounds showed strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 54.1-291.6 micro g/ear. Furthermore, at 1.0 and 0.1 mg/mouse of stevioside mixture, the mixture of these compounds markedly inhibited the promoting effect of TPA (1 micro g/mouse) on skin tumor formation initiated with 7,12-dimethylbenz[a]anthracene (50 micro g/mouse).

摘要

从甜叶菊中分离出了四种甜菊醇（贝壳杉烯型二萜）糖苷，即甜菊糖苷、莱鲍迪苷A和C以及杜克苷A。这些化合物对12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导的小鼠炎症表现出强烈的抑制活性。这些化合物对TPA诱导炎症的50%抑制剂量为54.1-291.6微克/耳。此外，在甜菊糖苷混合物剂量为1.0和0.1毫克/小鼠时，这些化合物的混合物显著抑制了TPA（1微克/小鼠）对由7,12-二甲基苯并[a]蒽（50微克/小鼠）引发的皮肤肿瘤形成的促进作用。