Jiang Jingle, Qi Lina, Lv Zengpeng, Jin Song, Wei Xihui, Shi Fangxiong
National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Changzhou Animal Disease Control Center, Bureau of Agriculture and Rural Affairs of Changzhou, Changzhou 213003, China.
Antioxidants (Basel). 2019 Nov 21;8(12):575. doi: 10.3390/antiox8120575.
The study was conducted to investigate the effects of dietary stevioside (STE) supplementation on the lipopolysaccharide (LPS)-induced intestinal mucosal damage of broiler chickens. A total of 192 one-day-old male Ross 308 broiler chicks were randomly divided into four treatments: (1) basal diet (CON); (2) basal diet supplemented with 250 mg/kg stevioside (STE); (3) basal diet + LPS-challenge (LPS); (4) basal diet supplemented with 250 mg/kg stevioside + LPS-challenge (LPS + STE). LPS-challenged groups received an intraperitoneal injection of LPS at 17, 19 and 21 d, whereas the CON and STE groups received a saline injection. The results showed that dietary STE supplementation normalized LPS-induced changes in protein expression of p-NF-κB and p-IκBα, mRNA expression of inflammatory genes (TLR4, NF-κB, and IFN-γ), tight junction-related genes (CLDN2, OCLN, and ZO-1), and antioxidant genes (Nrf2 and HO-1). LPS-induced decreases in serum diamine oxidase (DAO) level, villus height-to-crypt depth ratio, apoptotic index, and protein expression of proliferating cell nuclear antigen (PCNA) were reversed with dietary STE supplementation. Additionally, STE supplementation ameliorated the redox damage by reducing malondialdehyde (MDA) content and increasing total antioxidant capacity (T-AOC) and antioxidant enzyme activity. In conclusion, dietary stevioside supplementation could alleviate LPS-induced intestinal mucosal damage through anti-inflammatory and antioxidant effects in broiler chickens.
本研究旨在探讨日粮添加甜菊糖苷（STE）对脂多糖（LPS）诱导的肉鸡肠道黏膜损伤的影响。总共192只1日龄雄性罗斯308肉鸡随机分为4组处理：（1）基础日粮（CON）；（2）基础日粮添加250 mg/kg甜菊糖苷（STE）；（3）基础日粮+LPS攻毒（LPS）；（4）基础日粮添加250 mg/kg甜菊糖苷+LPS攻毒（LPS+STE）。LPS攻毒组在17、19和21日龄时腹腔注射LPS，而CON组和STE组注射生理盐水。结果表明，日粮添加STE使LPS诱导的p-NF-κB和p-IκBα蛋白表达、炎症基因（TLR4、NF-κB和IFN-γ）mRNA表达、紧密连接相关基因（CLDN2、OCLN和ZO-1）以及抗氧化基因（Nrf2和HO-1）的变化恢复正常。日粮添加STE逆转了LPS诱导的血清二胺氧化酶（DAO）水平降低、绒毛高度与隐窝深度比值降低、凋亡指数降低以及增殖细胞核抗原（PCNA）蛋白表达降低。此外，添加STE通过降低丙二醛（MDA）含量、增加总抗氧化能力（T-AOC）和抗氧化酶活性改善了氧化还原损伤。总之，日粮添加甜菊糖苷可通过抗炎和抗氧化作用减轻LPS诱导的肉鸡肠道黏膜损伤。
