Bahmed Karim, Bonaly Roger, Wathier Michel, Pucci Bernard, Coulon Joël
Université Henri Poincaré, Nancy 1, Faculté de Pharmacie, UMR UHP-CNRS 7564-LCPME Biochimie Microbienne, Nancy, France.
FEMS Microbiol Lett. 2002 Oct 29;216(1):99-103. doi: 10.1111/j.1574-6968.2002.tb11421.x.
The culture of two Kluyveromyces species, Kluyveromyces lactis (ATCC 96897) and Kluyveromyces bulgaricus (ATCC 96631), in the presence of subinhibitory doses of amphotericin B leads to the selection of mutants which are resistant to this polyene. The mutants show an alteration of their cell wall composition with the main change corresponding to an increase of chitin. The enzyme activities involved in the metabolism of this polymer, i.e. chitin synthases and chitinase, were measured. The results demonstrate that in both mutants the activity of chitinase was drastically decreased by 99% in comparison with the activity measured in the corresponding wild-type strain while no significant change of the chitin synthase I, II and III activities could be detected.
在亚抑制剂量的两性霉素B存在的情况下，对两种克鲁维酵母属酵母，即乳酸克鲁维酵母（ATCC 96897）和保加利亚克鲁维酵母（ATCC 96631）进行培养，会导致对这种多烯耐药的突变体的产生。这些突变体的细胞壁组成发生了改变，主要变化是几丁质增加。测定了参与这种聚合物代谢的酶活性，即几丁质合酶和几丁质酶。结果表明，与相应野生型菌株中测得的活性相比，两种突变体中的几丁质酶活性均急剧下降了99%，而未检测到几丁质合酶I、II和III活性有显著变化。
