King R J, Ruch J, Gikas E G, Platzker A C, Creasy R K
J Appl Physiol. 1975 Nov;39(5):735-41. doi: 10.1152/jappl.1975.39.5.735.
We have isolated and partially characterized the protein found in surface-active material from adult human lungs, and have determined the time of appearance of this protein in amniotic fluid. Fluids were drawn at gestational ages from 12 to 44 wk, and were assayed for their concentration of apoprotein by an agglutination immunoassay. Surfactant apoprotein ((defined as that protein which is reproducibly found in purified preparations of surface active material) was usually first detected from 30 to 32 wk gestation, and its concentration increased almost fivefold to a maximum at 37 wk. The change in apoprotein concentration was approximately paralleled by the change in phospholipid concentration. At all gestational ages there was wide variability in both phospholipid and apoprotein concentrations, and the time of appearance of the apoprotein in amniotic fluid differed among fetuses. The results suggest that the presence of surfactant apoprotein in amniotic fluid is coincident with the biochemical and morphological maturation of the fetal lung, and are additional evidence that this apoprotein is cosecreted with the lipids of surface-active material.
我们已从成人肺脏的表面活性物质中分离出一种蛋白质并对其进行了部分特性鉴定，还确定了这种蛋白质在羊水中出现的时间。在妊娠12至44周期间抽取羊水样本，通过凝集免疫测定法检测其中载脂蛋白的浓度。表面活性剂载脂蛋白（定义为在表面活性物质纯化制剂中可重复性检测到的蛋白质）通常在妊娠30至32周时首次被检测到，其浓度在37周时几乎增加了五倍达到最大值。载脂蛋白浓度的变化与磷脂浓度的变化大致平行。在所有孕周，磷脂和载脂蛋白浓度都存在很大差异，且不同胎儿羊水中载脂蛋白出现的时间也不同。结果表明，羊水中表面活性剂载脂蛋白的存在与胎儿肺脏的生化和形态成熟同时发生，这进一步证明这种载脂蛋白与表面活性物质的脂质是共同分泌的。
