摩根氏摩根菌O-特异性多糖的结构研究，其由两种高级糖组成。

Structural investigation of the O-specific polysaccharides of Morganella morganii consisting of two higher sugars.

作者信息

Kilcoyne Michelle, Shashkov Alexander S, Senchenkova Sof'ya A, Knirel Yuriy A, Vinogradov Evgeny V, Radziejewska-Lebrecht Joanna, Galimska-Stypa Regina, Savage Angela V

机构信息

Department of Chemistry, National University of Ireland, Galway, Ireland.

出版信息

Carbohydr Res. 2002 Oct 8;337(18):1697-702. doi: 10.1016/s0008-6215(02)00181-7.

DOI:10.1016/s0008-6215(02)00181-7

PMID:12423973

Abstract

The lipopolysaccharide of the bacterium Morganella morganii (strain KF 1676, RK 4222) yielded two polysaccharides, PS1 and PS2, when subjected to mild acid degradation followed by GPC. The polysaccharides were studied by 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, 1H,(13)C HMQC, and HMBC experiments. Each polysaccharide was found to contain a disaccharide repeating unit consisting of two higher sugars, 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid (a derivative of 8-epilegionaminic acid, 8eLeg5Am7Ac) and 2-acetamido-4-C-(3'-carboxamide-2',2'-dihydroxypropyl)-2,6-dideoxy-D-galactose (shewanellose, She). The two polysaccharides differ only in the ring size of shewanellose and have the following structures:Shewanellose has been previously identified in a phenol-soluble polysaccharide from Shewanella putrefaciens A6, which shows a close structural similarity to PS2.

摘要

摩根氏摩根菌（菌株KF 1676、RK 4222）的脂多糖经温和酸降解后再进行凝胶渗透色谱（GPC）分析，得到了两种多糖，即PS1和PS2。通过¹H和¹³C核磁共振波谱对这些多糖进行了研究，包括二维COSY、TOCSY、NOESY、¹H,(¹³)C HMQC和HMBC实验。发现每种多糖都含有一个由两种高级糖组成的二糖重复单元，即5-脒基-7-乙酰氨基-3,5,7,9-四脱氧-L-甘油-D-半乳糖壬-2-酮糖酸（8-表军团氨糖酸的衍生物，8eLeg5Am7Ac）和2-乙酰氨基-4-C-(3'-羧酰胺-2',2'-二羟基丙基)-2,6-二脱氧-D-半乳糖（希瓦氏糖，She）。这两种多糖仅在希瓦氏糖的环大小上有所不同，其结构如下：希瓦氏糖先前已在腐败希瓦氏菌A6的一种酚溶性多糖中被鉴定出来，它与PS2显示出密切的结构相似性。