Mehta U, Kang B P S, Kukreja R S, Bansal M P
Department of Biophysics, Panjab University, Chandigarh, India.
J Appl Toxicol. 2002 Nov-Dec;22(6):405-13. doi: 10.1002/jat.877.
Experiments were carried out to examine the changes occurring in the wall of rabbit aortae following high-fat diet (HFD) feeding as well as HFD + selenium supplementation. Male New Zealand White rabbits were divided into three groups-control, HFD-fed and HFD + Se supplementation-and were treated for three months. The study depicted that levels of serum total cholesterol and triglycerides were markedly increased in the HFD-fed group as compared with control animals. However, in the HFD + Se-fed group, these levels were markedly suppressed vis-à-vis animals fed on HFD only. Development of atherogenic and atheromatic plaques has been shown at the light microscopy level in HFD-fed rabbits, whereas these developments were not visible in the HFD + Se-fed rabbits. Transmission electron microscopy findings indicated altered ultrastructure in the endothelial cells of the intimal layer as well as smooth-muscle cells of the medial layer in HFD-fed animals. However, these findings indicated normal ultrastructure in most of the cells, with little ultrastructural alterations from animals supplemented with Se along with HFD feeding. The study on the whole depicted the ability of Se to inhibit the onset of progression of aortic disease and hence has relevance to its therapeutic potential.
进行了实验以研究高脂饮食（HFD）喂养以及HFD + 补充硒后兔主动脉壁发生的变化。雄性新西兰白兔分为三组——对照组、HFD喂养组和HFD + 硒补充组——并进行了三个月的处理。研究表明，与对照动物相比，HFD喂养组的血清总胆固醇和甘油三酯水平显著升高。然而，与仅喂食HFD的动物相比，HFD + 硒喂养组的这些水平显著降低。在光镜水平上，HFD喂养的兔子出现了动脉粥样硬化和动脉粥样斑块，而在HFD + 硒喂养的兔子中未观察到这些病变。透射电子显微镜检查结果表明，HFD喂养动物的内膜层内皮细胞以及中层平滑肌细胞的超微结构发生了改变。然而，这些结果表明大多数细胞的超微结构正常，与HFD喂养并补充硒的动物相比，超微结构改变很小。总体而言，该研究表明硒具有抑制主动脉疾病进展的能力，因此与其治疗潜力相关。
