Kang B P, Bansal M P, Mehta U
Department of Biophysics, Panjab University Chandigarh, India.
Gen Physiol Biophys. 1998 Mar;17(1):71-8.
Male Sprague Dawley rats were divided into three groups, viz (I) Controls, (II) High fat diet (HFD) fed, (III) HFD fed+selenium supplemented. After three months of treatment, there were significant increases in serum cholesterol and triglycerides in HFD fed group as compared to control. However, in Se supplemented group, the levels of serum cholesterol and triglycerides were significantly less as compared to group II. Selenium-dependent glutathione peroxidase (GSH-Px) activity in the liver and the aorta increased significantly in HFD fed animals and also showed additional significant increase on selenium supplementation. Malonyldialdehyde (MDA) concentrations in serum, liver and aorta and the activity of nitric oxide synthase (NOS; evident from reactive nitrogen intermediates and citrulline levels) in plasma showed significant increases in HFD fed group. However, supplementation of selenium led to a significant reduction in the levels of these parameters vis-a-vis HFD fed animals except in MDA levels in the serum and the liver where this decrease was non-significant. The important finding of this study is that selenium supplementation modulates the sequences favoring pathogenesis of atherosclerosis.
雄性Sprague Dawley大鼠被分为三组，即（I）对照组，（II）高脂饮食（HFD）喂养组，（III）高脂饮食喂养并补充硒组。治疗三个月后，与对照组相比，高脂饮食喂养组的血清胆固醇和甘油三酯显著升高。然而，在补充硒的组中，血清胆固醇和甘油三酯水平与第二组相比显著降低。高脂饮食喂养动物的肝脏和主动脉中硒依赖性谷胱甘肽过氧化物酶（GSH-Px）活性显著增加，补充硒后也显示出进一步的显著增加。高脂饮食喂养组的血清、肝脏和主动脉中的丙二醛（MDA）浓度以及血浆中的一氧化氮合酶（NOS；从活性氮中间体和瓜氨酸水平可明显看出）活性显著增加。然而，与高脂饮食喂养动物相比，补充硒导致这些参数水平显著降低，但血清和肝脏中的MDA水平除外，其降低不显著。本研究的重要发现是，补充硒可调节有利于动脉粥样硬化发病机制的一系列过程。
