Jin Hyoung-Joon, Fridrikh Sergey V, Rutledge Gregory C, Kaplan David L
Department of Chemical & Biological Engineering, Bioengineering Center, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA.
Biomacromolecules. 2002 Nov-Dec;3(6):1233-9. doi: 10.1021/bm025581u.
Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900,000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabrication of biomaterial scaffolds based on this unique fibrous protein.
用于形成纳米级直径纤维的静电纺丝技术已被用于探索高性能过滤器以及用于血管移植物或伤口敷料的生物材料支架。具有纳米级直径的纤维因其高表面积而具有优势。在本研究中，我们探索了用于基于蛋白质的生物材料的静电纺丝技术，以从家蚕（Bombyx mori）的再生蚕丝溶液中制备支架和膜。为了提高蛋白质溶液的可加工性，将分子量为900,000的聚环氧乙烷（PEO）与丝素蛋白混合。成功地对各种丝/ PEO水性混合物进行了静电纺丝。使用高分辨率扫描电子显微镜对纤维的形态进行了表征。纤维直径均匀且小于800nm。通过X射线光电子能谱估计组成，以表征丝/ PEO表面含量。基于这种独特的纤维状蛋白质，蚕丝和丝/ PEO混合物的水基静电纺丝为生物材料支架的制造提供了潜在有用的选择。
