Milyaeva Olga Yu, Rotanova Kseniya Yu, Rafikova Anastasiya R, Miller Reinhard, Loglio Giuseppe, Noskov Boris A
Department of Colloid Chemistry, St. Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
Institute of Condensed Matter Physics, Technische Universität Darmstadt, D-64289 Darmstadt, Germany.
Polymers (Basel). 2025 Jun 21;17(13):1733. doi: 10.3390/polym17131733.
Materials formed with a base of silk fibroin (SF) are successfully used in tissue engineering since their properties are similar to those of natural extracellular matrixes. Mixing SF with different polymers, for example, polyethylene oxide (PEO) and polyvinylpyrrolidone (PVP), allows the production of fibers, hydrogels, and films and their morphology to be controlled. The impact of PEO and PVP on formation and structure of SF adsorption layers was studied at different was studied at different polymer concentrations (from 0.002 to 0.5 mg/mL) and surface lifetimes. The protein concentration was fixed at 0.02 and 0.2 mg/mL. These concentrations are characterized by different types of spontaneously formed structures at the air-water interface. Since both synthetic polymers possess surface activity, they can penetrate the fibroin adsorption layer, leading to a decrease in the dynamic surface elasticity at almost constant surface tension and a decrease in ellipsometric angle Δ and adsorption layer thickness. As shown by AFM, the presence of polymers increases the porosity of the adsorption layer, due to the possible arrangement of protein and polymer molecules into separate domains, and can result in various morphology types such as fibers or tree-like ribbons. Therefore, polymers like PEO and PVP can be used to regulate the SF self-assembly at the interface, which in turn can affect the properties of the materials with high surface areas like electrospun matts and scaffolds.
以丝素蛋白（SF）为基础形成的材料已成功应用于组织工程，因为它们的特性与天然细胞外基质相似。将SF与不同的聚合物混合，例如聚环氧乙烷（PEO）和聚乙烯吡咯烷酮（PVP），可以制备纤维、水凝胶和薄膜，并控制它们的形态。研究了在不同聚合物浓度（0.002至0.5mg/mL）和表面寿命下，PEO和PVP对SF吸附层形成和结构的影响。蛋白质浓度固定在0.02和0.2mg/mL。这些浓度在气-水界面具有不同类型的自发形成结构。由于两种合成聚合物都具有表面活性，它们可以穿透丝素蛋白吸附层，导致在几乎恒定的表面张力下动态表面弹性降低，椭圆偏振角Δ和吸附层厚度减小。原子力显微镜（AFM）显示，聚合物的存在增加了吸附层的孔隙率，这是由于蛋白质和聚合物分子可能排列成单独的区域，并可能导致各种形态类型，如纤维或树状带。因此，PEO和PVP等聚合物可用于调节界面处的SF自组装，这反过来又会影响具有高表面积的材料（如电纺垫和支架）的性能。
