腺苷脱氨酶作用于RNA的编辑对于秀丽隐杆线虫的正常行为很重要。
RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans.
作者信息
Tonkin Leath A, Saccomanno Lisa, Morse Daniel P, Brodigan Thomas, Krause Michael, Bass Brenda L
机构信息
Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA.
出版信息
EMBO J. 2002 Nov 15;21(22):6025-35. doi: 10.1093/emboj/cdf607.
Abstract
Here we take advantage of the well-characterized and simple nervous system of Caenorhabditis elegans to further our understanding of the functions of RNA editing. We describe the two C.elegans ADAR genes, adr-1 and adr-2, and characterize strains containing homozygous deletions in each, or both, of these genes. We find that adr-1 is expressed in most, if not all, cells of the C.elegans nervous system and also in the developing vulva. Using chemotaxis assays, we show that both ADARs are important for normal behavior. Biochemical, molecular and phenotypic analyses indicate that ADR-1 and ADR-2 have distinct roles in C.elegans, but sometimes act together.
摘要
在这里，我们利用模式生物秀丽隐杆线虫特征明确且简单的神经系统，来进一步理解RNA编辑的功能。我们描述了线虫的两个ADAR基因，adr-1和adr-2，并对这些基因中单个或两个基因均存在纯合缺失的菌株进行了特征分析。我们发现adr-1在秀丽隐杆线虫神经系统的大部分（如果不是全部）细胞以及发育中的阴门中均有表达。通过趋化性分析，我们表明这两种ADAR蛋白对正常行为都很重要。生化、分子和表型分析表明，ADR-1和ADR-2在秀丽隐杆线虫中具有不同的作用，但有时也会共同发挥作用。
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